KMID : 0900919990230030257
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Korean journal of Animal Reproduction 1999 Volume.23 No. 3 p.257 ~ p.262
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A Study on Efficiency of Boar Semen Extender KpA for Swine AI Aspects of Storage Temperature and pH Change
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Chung Koo-Min
Lim Kyung-Sun
Shin Young-Soo Kim I. C. Lee Chang-Hee
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Abstract
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This study was carried out to evaluate the effect of pH and storage temperature on the viability of boar sperm diluted with extender KpA for swine artificial insemination. The results obtained in this experiment were summarized as follows : 1. The viability of boar sperm diluted with KpA was higher than that with BTS during storage and especially more than 60% of sperm viability in KpA was maintained through 6 days. The pH values of all extenders were kept during storage of semens following dilution. 2. The sperm diluted with acidic (pH 6.3~6.8) or alkalic (pH 7.8~8.0) KpA and stored at 4^{circ}C or 37^{circ}C were more sensitive in viability than that with neutral pH (6.8~7.3) and at 17^{circ}C storage. But pH values of all conditions were not increased rapidly. Especially acidic and alkalic diluents were more stable in pH after dilution. Conclusively, extender pH and storage temperature was the important factors for sperm viability. The KpA setting up around neutral pH was the optimal boar semen extender for maintaining liquid semen at 17^{circ}C.
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KEYWORD
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KpA, Extender, pH, Boar semen, Sperm viability
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